Human IFN-γ Flex Set
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產(chǎn)品英文名稱:Human IFN-γ Flex Set
產(chǎn)品中文名稱:人干擾素-γ 伸縮裝置
Human IFN-γ Flex Set 產(chǎn)品介紹
The BD™ CBA Human IFN-γ Flex Set is a bead-based immunoassay capable of measuring human interferon-γ (IFN-γ) in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human IFN-γ Flex Set. The biology and function of IFN-γ has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).
人干擾素-γ Flex set 的成分
- Capture Antibody: Anti-human IFN-γ
- Detection Antibody: Biotinylated anti-human IFN-γ
- Enzyme Reagent: Streptavidin-horseradish peroxidase conjugate (SAv-HRP)
- Standard: Recombinant human IFN-γ (lyophilized)
人干擾素-γ Flex set 的優(yōu)越性
•高靈敏度
•高通量,高含量的分析
•最小體積的生物材料的要求
•適用于冷凍/解凍的生物樣品 •與其他檢驗(yàn)方法兼容。例如,細(xì)胞分析。BD™ ELISPOT可以轉(zhuǎn)移克隆,增殖實(shí)驗(yàn),流式細(xì)胞術(shù)或其他分析方法。
Human IFN-γ Flex Set 的保存方法
This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
Human IFN-γ Flex Set 推薦檢驗(yàn)步驟
The BD CBA Human IFN-γ Flex Set must be used in conjunction with a BD CBA Human Soluble Protein
Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488).Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IFN-γ Flex Set will be 2,500 pg/ml.
The BD CBA Human IFN-γ Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents.
人干擾素-γ Flex set 使用注意事項(xiàng)
1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
2. ProClin is a trademark of Rohm and Haas Company.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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