產(chǎn)品英文名稱：Mouse Hematopoietic Stem and ProgenitorCell Isolation Kit
產(chǎn)品中文名稱：小鼠造血干細(xì)胞和祖細(xì)胞分離試劑盒

BD Stemflow™ Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit的簡介
試劑盒提供必要試劑借助多色流式細(xì)胞儀從小鼠骨髓樣品分離造血祖細(xì)胞（HCPs）和造血干細(xì)胞（HSCs）。小鼠HCPs和HSCs具備一組細(xì)胞表面標(biāo)志物，以及具有高水平的染料外排活性。BD小鼠造血干細(xì)胞和祖細(xì)胞分離試劑盒包含特異性標(biāo)志物的熒光抗體和有助于識別HPCs和HSCs的熒光抗體。試劑盒中含有的特異性抗體包括：CD3的抗體（T細(xì)胞標(biāo)志物），CD45R(B220)(B細(xì)胞標(biāo)志物)，Ly6C和Ly6G(Gr1)(粒細(xì)胞標(biāo)志物)，CD11b(Mac1)(巨噬細(xì)胞標(biāo)志物)，以及TER-119(紅細(xì)胞標(biāo)志物)。試劑盒中同時含有3種特異性抗體用于分離HPCs和HSCs。第一個是CD34，它由HPCs表達(dá)，在干細(xì)胞中為陰性。另外兩個是Sca-1和c-Kit(CD117)，它們由HPCs和HSCs表達(dá)。使用這些抗體去分離祖細(xì)胞和干細(xì)胞（稱為CD34-/dim KSL細(xì)胞，KSL為c-Kit+/Sca-1+/Lineage-），這種分離方法已被廣泛報道。每種特異性抗體都有非特異性同型對照。另外還含有7-AAD和BD CompBead 補(bǔ)償顆粒。具體操作方法詳見說明書。

Mouse Hematopoietic Stem and ProgenitorCell Isolation Kit 的物質(zhì)組成
CD34 FITC, c-Kit PE, Sca-1 PE-Cy™7 and Lineage Cocktail APC; isotype controls; 7-AAD; BD™ CompBead compensation particles.

BD Stemflow™ Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit的保存條件
The entire BD Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit must be stored in the dark at 2–8°C. Do not freeze.

BD Stemflow™小鼠造血干細(xì)胞和祖細(xì)胞分離試劑盒的特征
1.Enables high purity isolation of HPC and HSC subpopulations from mouse bone marrow
2.Delivers a streamlined solution for consistentexperiments with fluorochrome-conjugated antibodies,compensation beads, protocols, and analysis guidelines
3.Facilitates compensation and scatter setup and conserves
cells with BD CompBead microparticles
4.Provides flexibility to drop in additional fluorochromeconjugated antibodies. Compatible with side population staining and upfront magnetic bead enrichment.

BD Stemflow™小鼠造血干細(xì)胞和祖細(xì)胞分離試劑盒的特別注意事項
The reagents in this kit contain sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.