MycoAlert? PLUS Mycoplasma Detection Kit
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產(chǎn)品英文名稱:MycoAlert? PLUS Mycoplasma Detection Kit
產(chǎn)品中文名稱:MycoAlert? PLUS支原體檢測(cè)試劑盒
MycoAlert? PLUS支原體檢測(cè)試劑盒 產(chǎn)品介紹:
MycoAlert? PLUS Assay是一種精選生物化學(xué)檢測(cè)方法,用于檢測(cè)細(xì)胞培養(yǎng)過程中的支原體污染問題??梢詸z測(cè)出所有6種支原體屬,多達(dá)180種支原體。檢測(cè)樣品中的活性支原體裂解后,釋放出的酶與MycoAlert? PLUS Substrate反應(yīng),催化ADP轉(zhuǎn)化為ATP。在MycoAlert? PLUS Reagent中在熒光素酶作用下ATP產(chǎn)生光信號(hào)。光信號(hào)可用光度計(jì)讀出,根據(jù)第二次讀數(shù)與第一次讀數(shù)比率可以確定是否存在支原體污染。比率<1, 支原體陰性,無支原體;1-1.2,不確定是否存在支原體,需重新檢測(cè);>1.2,支原體陽性,存在支原體污染。
? 使用方便:只需向細(xì)胞上清液樣品中添加兩種試劑,兩次光度計(jì)讀數(shù)
? 檢測(cè)快速:20分鐘就可得到檢測(cè)結(jié)果,結(jié)果分析簡(jiǎn)單方便
? 通用性強(qiáng):可檢測(cè)所有常見的支原體污染(支原體屬,無膽甾原體屬和蟲原體屬,不包括脲原體屬),適合使用試管光度計(jì),板光度計(jì)和多功能讀數(shù)器
MycoAlert? PLUS Mycoplasma Detection Kit:
The MycoAlert? PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert? PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal? via the luciferase enzyme in the MycoAlert? PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert? PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
訂購(gòu)信息:
? LT07-701 MycoAlert? PLUS Mycoplasma Detection Kit, 10Tests
? LT07-703 MycoAlert? PLUS Mycoplasma Detection Kit, 30Tests
? LT07-705 MycoAlert? PLUS Mycoplasma Detection Kit, 50Tests
? LT07-710 MycoAlert? PLUS Mycoplasma Detection Kit, 100Tests
? LT07-518 MycoAlert? Assay Control Sets, 10Tests
? LT27-292 MycoAlert? Assay Buffer, 20ml
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