Insect-XPRESS? Protein-free Insect Cell Medium with L-glutamine
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產(chǎn)品英文名稱:Insect-XPRESS? Protein-free Insect Cell Medium with L-glutamine
產(chǎn)品中文名稱:Insect-XPRESS?無蛋白昆蟲細(xì)胞培養(yǎng)基,含L-谷氨酰胺
Insect-XPRESS?培養(yǎng)基 產(chǎn)品介紹:
Insect-XPRESS?培養(yǎng)基不含蛋白成份,設(shè)計(jì)用于培養(yǎng)從草地夜蛾(Sf9 and Sf21)分離的昆蟲細(xì)胞系。使用Insect-XPRESS?培養(yǎng)基進(jìn)行Sf9細(xì)胞懸浮通氣培養(yǎng)可以收獲超過8.3 × 106個(gè)細(xì)胞/mL。Insect-XPRESS? Medium也可用于細(xì)胞固定化單層培養(yǎng)和搖瓶培養(yǎng)。培養(yǎng)基中含有L-谷氨酰胺,可用于在桿狀病毒表達(dá)系統(tǒng)(BEVS)中表達(dá)的重組蛋白產(chǎn)物培養(yǎng)。對(duì)于培養(yǎng)冷凍昆蟲細(xì)胞,Insect-XPRESS?可以和防冷凍培養(yǎng)基(Cat. No. 12-132)按50:50混合使用。
Insect-XPRESS? Medium不含有胰蛋白酶抑制劑,如果使用胰蛋白酶釋放細(xì)胞,建議馬上進(jìn)行離心分離并轉(zhuǎn)移到新培養(yǎng)基中。
Insect-XPRESS? Protein-free Insect Cell Medium with L-glutamine英文簡(jiǎn)述:
Insect-XPRESS? Medium is a protein-free formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). Cell densities in excess of 8.3 × 106 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS? Medium and an excess of oxygen. This formulation can also be used for stationary mono?layer cultures and shake-flask cultures. Insect-XPRESS? Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS). For cryopreservation of insect cells, Insect-XPRESS? can be mixed 50:50 with Cryoprotective Medium (Cat. No. 12-132).
訂購信息:
12-730F Insect-XPRESS? Protein-free Insect Cell Medium with L-glutamine 500 ml
17-730Q Insect-XPRESS? Protein-free Insect Cell Medium with L-glutamine 1 L
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