產(chǎn)品英文名稱(chēng)：C-peptide ELISA Kit
產(chǎn)品中文名稱(chēng)：C-肽ELISA檢測(cè)試劑盒

§ 檢測(cè)原理：ALPCO C-肽ELISA是一個(gè)夾層式免疫檢測(cè)試劑盒。96孔微孔板涂有一層C-肽特異性單克隆抗體。將標(biāo)準(zhǔn)物，對(duì)照品和樣品一并添加到微型板小孔中與檢測(cè)緩沖液混合。然后將微孔板放在搖床上室溫溫育1小時(shí)，700－900轉(zhuǎn)/分鐘。第一次溫育結(jié)束，用清洗緩沖液清洗小孔并烘干。添加結(jié)合物（配制方法參照說(shuō)明書(shū)），微孔板放在搖床上第二次室溫溫育1小時(shí)，700－900轉(zhuǎn)/分鐘。添加TMB作用底物，放在微孔板搖床上室溫溫育15分鐘，700－900轉(zhuǎn)/分鐘。第三次溫育結(jié)束，添加反應(yīng)終止液，調(diào)節(jié)分光光度計(jì)到450nm測(cè)定OD值。由于發(fā)光強(qiáng)度與樣品中C-肽含量成比例，因此可以根據(jù)OD值確定C-肽含量。

§ 檢測(cè)過(guò)程：各添加25μl標(biāo)準(zhǔn)物，對(duì)照物和樣品→加50µl檢測(cè)緩沖液→微孔板搖床室溫溫育1小時(shí)，700－900rpm→清洗緩沖液清洗6次→加100µl結(jié)合物（配制方法參照說(shuō)明書(shū)）→微孔板搖床室溫溫育1小時(shí)，700－900rpm→清洗緩沖液清洗6次→添加100µl TMB作用底物→微孔板搖床室溫溫育15分鐘，700－900rpm→添加100µl反應(yīng)終止液→分光光度計(jì)450nm讀數(shù)

ALPCO C-peptide ELISA kit英文簡(jiǎn)述：
The ALPCO C-peptide ELISA kit is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

INTENDED USE：The ALPCO C-peptide ELISA is designed for the quantitative determination of C-peptide in human serum and plasma.

PRINCIPLE OF THE ASSAY：The ALPCO C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the Assay Buffer. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The Conjugate is then added, and the microplate is incubated a second time on a microplate shaker at 700-900 rpm, washed, and blotted dry. TMB Substrate is added, and the microplate is incubated a third time at room temperature on a microplate shaker at 700-900 rpm. Once the third incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.