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產(chǎn)品編號：HY-001459

BioGems 人RANTES 預(yù)包被ELISA試劑盒,Human RANTES Pre-Coated ELISA kit

Human RANTES Pre-Coated ELISA kit

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  • 生產(chǎn)廠家： BioGems
  • 訂購貨號：
  • 規(guī)格型號： 1 Kit
  • 產(chǎn)品編號： HY-001459
  • 產(chǎn)品種類： 細(xì)胞活力檢測

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產(chǎn)品描述 說明書【下載】 用戶評論【0】

RANTES是CC趨化因子，可通過CCR1，CCR3，CCR5和US28（巨細(xì)胞病毒受體）受體傳導(dǎo)信號。 它是一種趨化單核細(xì)胞，記憶T細(xì)胞（CD4 + / CD45RO），嗜堿性粒細(xì)胞和嗜酸性粒細(xì)胞的趨化劑。RANTES還具有抑制某些HIV-1，HIV-2和猿免疫缺陷病毒（SIV）的能力。

BioGems人類RANTES預(yù)包被ELISA（酶聯(lián)免疫吸附測定）試劑盒采用固相免疫測定法，專門設(shè)計(jì)用于檢測RANTES，96孔板預(yù)包被RANTES特異性小鼠單克隆抗體。它含有大腸桿菌表達(dá)的重組RANTES。檢測抗體是RANTES特異性的生物素化山羊多克隆抗體。該試劑盒已經(jīng)過分析驗(yàn)證，是即用型試劑。

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Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<3pg/ml

Range:

15.6pg/ml-1000pg/ml

Specificity:

Natural and recombinant Human Rantes

Uniprot:

D0EI67

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 – 8 ℃.

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Components

Description

Quantity

Volume

Anti-Human Rantes Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human Rantes Standard

2

10 ng vial

Human Rantes Biotinylated antibody (100x)

1

130 μL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 μL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

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Reproducibility

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Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

147

293

501

110

339

658

Standard Deviation

5.88

15.53

31.1

8.14

24.41

56.6

Consistency (%CV)

4

5.3

6.2

7.4

7.2

8.6

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Description

RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).

The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.

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