Human MIP-3β (CCL19) Pre-Coated ELISA Kit
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MIP-3β是一種CC趨化因子,在胸腺,淋巴結(jié)和活化的骨髓基質(zhì)細(xì)胞中表達(dá),通過(guò)CCR7受體傳導(dǎo)。 MIP-3β是T和B淋巴細(xì)胞和骨髓祖細(xì)胞的趨化因子。
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BioGems人MIP-3β(CCL19)預(yù)包被ELISA(酶聯(lián)免疫吸附測(cè)定)試劑盒采用固相免疫法,專門設(shè)計(jì)用于檢測(cè)MIP-3β(CCL19),96孔微板預(yù)包被MIP-3β特異鼠單克隆抗體(CCL19)。它含有大腸桿菌表達(dá)的重組MIP-3β(CCL19)。檢測(cè)抗體是MIP-3β特異生物素化山羊多克隆抗體(CCL19)。該試劑盒是即用型試劑盒,已經(jīng)過(guò)分析驗(yàn)證。
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Human MIP-3β (CCL19) Pre-Coated ELISA Kit分析
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Product Details
Assay Type: |
Solid Phase Sandwich ELISA: 96-Well Strip Plate |
Sensitivity: |
<10pg/ml |
Range: |
15.6pg/ml-1000pg/ml |
Specificity: |
Natural and recombinant Human MIP-3β?(CCL19) |
Uniprot: |
Q6IBD6 |
Interference: |
No detectable cross-reactivity with other relevant proteins |
Storage Conditions: |
Store the unopened product at 2 – 8 ℃. |
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Components組份
Description |
Quantity |
Volume |
Anti-Human MIP-3β(CCL19) Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human MIP-3β?(CCL19) Standard |
2 |
10 ng vial |
Human MIP-3β?(CCL19) Biotinylated antibody (100x) |
1 |
130 μL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 μL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
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Reproducibility?可重復(fù)性
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Intra-Assay |
Inter-Assay |
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
97 |
293 |
583 |
117 |
374 |
627 |
Standard Deviation |
5.53 |
14.1 |
36.15 |
7.5 |
18.7 |
42.64 |
Consistency (%CV) |
5.7 |
4.8 |
6.2 |
6.4 |
5 |
6.8 |
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Catalog Number : BGK6IBD6
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Description
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β?is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Human MIP-3β?(CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β?(CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β?(CCL19). It contains E. coli-expressed recombinant MIP-3β?(CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β?(CCL19). The kit is analytically validated with ready to use reagents.
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