Fas和Fas配體（FasL）屬于TNF超家族，分別是I型和II型跨膜蛋白。FasL與Fas的結(jié)合觸發(fā)攜帶Fas細(xì)胞的凋亡。凋亡機(jī)制涉及通過稱為FADD的adaptor分子募集pro-caspase 8蛋白酶，隨后將酶原處理成活性形式。然后這些活性caspases蛋白酶切割各種細(xì)胞底物，導(dǎo)致最終的細(xì)胞死亡。sFasR能夠通過充當(dāng)FasL誘發(fā)的decoy受體來抑制FasL誘導(dǎo)的細(xì)胞凋亡（sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL）。

BioGems人類FAS預(yù)包被ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒是一種固相免疫測(cè)定法，專門設(shè)計(jì)用于測(cè)量FAS，其帶有FAS特異預(yù)包被鼠單克隆抗體的96孔微孔板。它含有NS0表達(dá)的重組FAS。檢測(cè)抗體是FAS特異的生物素化山羊多克隆抗體。該試劑盒是即用型試劑盒，已經(jīng)過分析驗(yàn)證。

Human FAS Pre-Coated ELISA試劑盒分析

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Product Details

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Components組份

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Reproducibility重復(fù)性

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Catalog Number : BGK25445

Description

Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.

The BioGems Human FAS Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure FAS with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FAS. It contains NS0-expressed recombinant FAS. The detection antibody is a biotinylated goat polyclonal antibody specific for FAS. The kit is analytically validated with ready to use reagents.