英文名稱：Recombinant Human Fibroblast Growth Factor-acidic
中文簡稱：【FGF 1】重組人酸性成纖維細(xì)胞生長因子

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重組人酸性成纖維細(xì)胞生長因子產(chǎn)品描述：

酸性成纖維細(xì)胞生長因子(acidic fibroblast growth factor，aFGF) 是成纖維細(xì)胞生長因子家族成員之一,對中胚層及神經(jīng)外胚層來源的細(xì)胞具有促分裂的作用。aFGF主要分布于大腦,它以自分泌或旁分泌的方式作用于周圍細(xì)胞,但其本身缺乏信號肽結(jié)構(gòu)。aFGF蛋白由154個氨基酸殘基組成，等電點5～7，通過與其受體結(jié)合啟動信號轉(zhuǎn)導(dǎo)機制從而誘導(dǎo)多種細(xì)胞分化、增殖。
人酸性成纖維細(xì)胞生長因子重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有141個氨基酸，分子量為15967Da。該人酸性成纖維細(xì)胞生長因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---大腸桿菌中重組表達(dá)

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重組人酸性成纖維細(xì)胞生長因子產(chǎn)品使用須知：
溶解：提供的人酸性成纖維細(xì)胞生長因子(FGF 1)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人酸性成纖維細(xì)胞生長因子(FGF 1)的10mM 磷酸鈉（pH 7.5)和75mM NaCl溶液中過濾滅菌并凍干產(chǎn)生的。我們建議加無菌的4℃ 18MΩ-cm水以配制工作儲存液到0.1-0.25mg/ml。將樣本在4℃中豎直放置5分鐘后，離心去除沉淀物。
保存：人酸性成纖維細(xì)胞生長因子(FGF 1)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Human Fibroblast Growth Factor-acidic 【FGF 1】重組人酸性成纖維細(xì)胞生長因子產(chǎn)品英文描述：

Synonyms 

HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Introduction 

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
Three alternatively spliced variants encoding different isoforms have been described.
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description 

Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of approximately 15.8kDa.
The FGF acidic is purified by proprietary chromatographic techniques.

Source 

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing PBS, pH 7.4.

Solubility   （溶解性）

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O at 4 degrees Celsius at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.

Stability    （穩(wěn)定性）

Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity      （純度）

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence    （氨基酸序列）

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Biological Activity     （生物活性）

The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is <0.5 ng/ml, corresponding to a specific activity of > 2.0 x 1,000,000IU/mg.