英文名稱：Recombinant Human Vascular Endothelial Growth Factor，CHO
中文簡稱：【VEGF CHO】重組人血管內(nèi)皮生長因子,CHO表達

Recombinant Human Vascular Endothelial Growth Factor，CHO 【VEGF CHO】重組人血管內(nèi)皮生長因子,CHO表達 細胞因子產(chǎn)品描述：

血管內(nèi)皮生長因子（Vascular endothelial growth factor，VEGF）是血管內(nèi)皮細胞特異性的肝素結(jié)合生長因子（heparin-binding growth factor），可在體內(nèi)誘導(dǎo)血管新生。VEGF有六種不同亞型，包括VEGF-A，-B，-C，-D，及-E；其分子量從35至44kDa不等，每個亞型特異性地與三個“血管內(nèi)皮生長因子受體”（VEGFR-1，-2，及-3)的特定組合相結(jié)合。VEGF是高度保守的同源二聚體糖蛋白。二條分子量各為24kDa的單鏈以二硫鍵組成二聚體。VEGF分解的單體無活性,去除N 2糖基對生物效應(yīng)無影響，但可能在細胞分泌中起作用。由于mRNA不同的剪切方式，產(chǎn)生出分別VEGF121、VEGF145、VEGF165、VEGF185、VEGF206等至少5種蛋白形式，其中VEGF121、VEGF145、VEGF165是分泌型可溶性蛋白，能直接作用于血管內(nèi)皮細胞促進血管內(nèi)皮細胞增殖，增加血管通透性。
人血管內(nèi)皮生長因子，CHO表達重組蛋白是在CHO中國倉鼠卵巢細胞中重組表達的，是由兩條糖基化多肽鏈構(gòu)成的同源二聚體。包含有165個氨基酸殘基。由于發(fā)生了糖基化修飾，其在SDS-PAGE電泳顯示分子量為44kDa。該人血管內(nèi)皮生長因子，CHO表達重組蛋白最終由標準的色譜技術(shù)純化出。
來源宿主---CHO中國倉鼠卵巢細胞中重組表達

Recombinant Human Vascular Endothelial Growth Factor，CHO 【VEGF CHO】重組人血管內(nèi)皮生長因子,CHO表達 細胞因子產(chǎn)品使用須知：

溶解：提供的人血管內(nèi)皮生長因子,CHO表達(VEGF CHO)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含人血管內(nèi)皮生長因子,CHO表達(VEGF CHO)的磷酸鹽緩沖鹽水（pH 7.4）中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到不低于100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存：人血管內(nèi)皮生長因子,CHO表達(VEGF CHO)在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Human Vascular Endothelial Growth Factor，CHO 【VEGF CHO】重組人血管內(nèi)皮生長因子,CHO表達 細胞因子產(chǎn)品英文描述：

Synonyms 

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction 

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ). VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description 

Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in SDS-PAGE under non-reducing conditions.
The VEGF is purified by proprietary chromatographic techniques.

Source 

Chinese Hamster Ovarian Cell.

Physical Appearance  （外觀）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a Phosphate- Buffered Saline, pH 7.4.

Solubility    （溶解性）

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability     （穩(wěn)定性）

Lyophilized Vascular Endothelial Growth Factor Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity     （純度）

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity     （生物活性）

Determined by its ability to stimulate 3H-Thymidine incorporation in human ublical vein endothelial cells, the ED50 for this effect was found to be 2-6ng/ml, corresponding to a Specific Activity of 166,667-500,000IU/mg.

Usage 

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.