Recombinant Human Interleukin-1 alpha
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英文名稱:Recombinant Human Interleukin-1 alpha
中文簡稱:【IL 1 alpha】重組人白介素-1α
Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重組人白介素-1α 細(xì)胞因子產(chǎn)品描述:
白細(xì)胞介素-1(interleukin-1,IL-1)是在感染和炎癥狀態(tài)下,由多種細(xì)胞產(chǎn)生的、有多方面生物學(xué)功能的細(xì)胞因子,其生物學(xué)作用十分廣泛,主要包括參與介導(dǎo)炎癥反應(yīng)、調(diào)節(jié)免疫、調(diào)節(jié)機(jī)體代謝。IL-1分子重量為17.5KD,有兩種類型:IL-1α和IL-1β,主要以IL-1β為主。IL-1受體(interleukin-1 receptor,IL-1R)包括Ⅰ型和Ⅱ型,此外還存在一種內(nèi)源性IL-1受體拮抗劑 (interleukin-1 receptor antagonist,IL-1ra或IL-1γ),IL-1ra可以與IL-1受體結(jié)合,但是不引起相應(yīng)的作用。
人白介素-1α重組蛋白是在大腸桿菌中重組表達(dá)的,是一條單鏈、非糖基化的多肽鏈,包含有159個(gè)氨基酸,分子量為18022Da。該人白介素-1α重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重組人白介素-1α 細(xì)胞因子產(chǎn)品使用須知:
溶解:提供的人白介素-1α(IL 1 alpha)產(chǎn)品為過濾滅菌的白色凍干粉末;該凍干粉末是從含1mg/ml人白介素-1α(IL 1 alpha)的20mM Tris-HCL(pH 8),5mM MgCl2 和10%甘油溶液中過濾滅菌并凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml,后繼實(shí)驗(yàn)過程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存:人白介素-1α(IL 1 alpha)凍干粉末在室溫可保存3周,或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延長保存時(shí)間,建議分裝后置于-18℃凍存。請避免反復(fù)凍融。
Recombinant Human Interleukin-1 alpha 【IL 1 alpha】重組人白介素-1α 細(xì)胞因子產(chǎn)品英文描述:
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Introduction
IL-1 alpha is produced by activated macrophages, stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1A proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Description
Interleukin-1 alpha Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton.
The IL-1A is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance (外觀)
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 25mM Tris-HCl, pH 8.
Solubility (溶解性)
It is recommended to reconstitute the lyophilized Interleukin 1 alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability (穩(wěn)定性)
Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1a should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity (純度)
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence (氨基酸序列)
SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAV KFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITG SETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILE NQA.
Biological Activity (生物活性)
The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a Specific Activity of 1 x 109 IU/mg.
Protein content (蛋白質(zhì)含量)
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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