英文名稱：Recombinant Human Erythropoietin-alpha Fc/Chimera

中文簡稱：【EPO a Fc】重組人紅細胞生成素-α受體/嵌合體

Recombinant Human Erythropoietin-alpha Fc/Chimera 【EPO a Fc】重組人紅細胞生成素-α受體/嵌合體 細胞因子描述：

紅細胞生成素（erythropoietin，EPO）對紅細胞生成的特異性刺激作用的細胞因子。紅細胞生成素是一種糖蛋白，在胎兒體內(nèi)由腎臟及肝臟產(chǎn)生，而在成人體內(nèi)主要由腎臟產(chǎn)生。目前已知人紅細胞生成素有兩種存在形式，即人EPO-α及人EPO-β，二者氨基酸組成及順序相同，都含有165個氨基酸殘基。分子量、等電點及生物活性也都類似，差別在于二者的糖型組成不同，EPO-α含有較多的N-乙酰氨基葡萄糖和N-乙酰神經(jīng)氨酸，總的含糖量也較EPO-β高。紅細胞生成素-α是調(diào)節(jié)紅細胞增殖、成熟和分化的一種造血生長因子。

人紅細胞生成素-α Fc/嵌合體重組蛋白是在CHO中國倉鼠卵巢細胞中重組表達的，是由兩條糖基化多肽鏈組成的二聚物，其中兩條成熟的人EPO分子連接到人IgG1的Fc部位。由于發(fā)生了糖基化，該重組嵌合體蛋白在非變性SDS-PAGE中的顯示分子量為140kDa。

CHO中國倉鼠卵巢細胞中重組表達

Recombinant Human Erythropoietin-alpha Fc/Chimera 【EPO a Fc】重組人紅細胞生成素-α受體/嵌合體 細胞因子實驗須知：

溶解：提供的人紅細胞生成素-α受體/嵌合體(EPO a Fc)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末中每毫克人紅細胞生成素-α受體/嵌合體是從1x PBS（pH 7.4）溶液中過濾并凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。

保存：人紅細胞生成素-α受體/嵌合體(EPO a Fc)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復凍融。

Recombinant Human Erythropoietin-alpha Fc/Chimera 【EPO a Fc】重組人紅細胞生成素-α受體/嵌合體 細胞因子英文描述：

Introduction 

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Description 

Erythropoietin-alpha Fc-Chimera Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a dimeric, glycosilated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.

Source 

Chinese Hamster Ovary Cells(CHO).

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

Each mg of lyophilized powder contains 1x PBS pH-7.4.

Solubility 

It is recommended to reconstitute the lyophilized Erythropoietin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability 

Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity 

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity 

The ED50 as determined by the dose-dependent stimulation of human megakaryoblastic leukemia cells is less than 2.0 ng/ml, corresponding to a Specific Activity of 5.0 x 105 IU/mg.

Usage 

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.