產(chǎn)品英文名稱：SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*
產(chǎn)品中文名稱：SensoLyte®620 HCV 蛋白酶熒光檢測試劑盒

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 產(chǎn)品介紹
丙型肝炎病毒（HCV）NS3／4A蛋白酶是病毒在NS3-NS4A，ns4a-ns4b，ns4b-ns5a，和ns5a-ns5b這些位點非結(jié)構(gòu)蛋白裂解所需的。這些裂解的病毒蛋白的成熟至關(guān)重要。因此，該蛋白酶成為開發(fā)抗HCV藥物的關(guān)鍵目標之一。該SensoLyte® 620 HCV蛋白酶檢測試劑盒提供了高流率的一個方便的測定。HCV NS3/4A蛋白酶抑制劑和HCV NS3/4A連續(xù)定量篩選使用Hilyte氟蛋白酶活性TR/QXL™610熒光共振能量轉(zhuǎn)移（FRET）肽。這個肽序列是來自裂解位點NS4A / NS4B。在苦惱的肽，HiLyte Fluor™r熒光淬滅通過QXL™610來完成。在分裂成兩個獨立的片段的HCV NS3/4A蛋白酶（方案1），HiLyte Fluor™TR是可以回收的，可監(jiān)測激發(fā)/發(fā)射= 591 nm和622 nm。與附近的紅色波長，Hilyte  Fluor™TR信號避免大多數(shù)測試化合物的干擾，因此是理想的流率HCV NS3/4A蛋白酶抑制劑的篩選。

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 的組分
Component A    HCV NS3/4A protease substrate                                      120 μL
                           Ex/Em=591 nm/622 nm upon cleavage
Component B    HiLyte Fluor™TR, fluorescence reference standard      100 μM, 5 μL
                           Ex/Em=591 nm/622 nm
Component C    2X Assay buffer                                                                  10 mL
Component D    Stop solution                                                                      10 mL
Component E    DTT                                                                                    1 M, 0.5 mL
Component F    Pep4AK                                                                               50 μL, 600 μM
                           HCV NS3 protease cofactor                         

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 的保存
• Store all kit components at -20°C
• Protect Components A and B from light
• Components C and D can be stored at room temperature for convenience

SensoLyte® 620 HCV 蛋白酶熒光檢測試劑盒的英文描述
The SensoLyte® 620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of Hilyte Fluor™ TR is quenched by QXL™ 610. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of Hilyte Fluor™ TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. With near red emission wavelength, this HiLyte Fluor™ TR/QXL™ 610-based FRET peptide avoids the autofluorescence interference from most test compounds. This feature makes this FRET peptide an ideal substrate for screening HCV protease inhibitors since some of screening compounds have strong autofluorescence at shorter wavelength, making other FRET peptide substrates inappropriate. The assays are performed in a convenient 96-well or 384-well microplate format.
The kit contains:
• HiLyte Fluor™ TR/QXL™ 610-based FRET peptide substrate (Ex/Em=590/620 nm upon cleavage)
• Assay buffer
• Fluorescence reference standard for calibration
• A detailed protocol
Kit Size: 100 assays

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 系列產(chǎn)品訂購信息
AnaSpec  71146  SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*  1 kit         
AnaSpec  72169-5  HCV NS3/4A protease genotype 4d, recombinant  5 µg            
AnaSpec  72168-5  HCV NS3/4A protease genotype 3a, recombinant  5 µg             
AnaSpec  72166-5  HCV NS3/4A protease genotype 1a, recombinant  5 µg             
AnaSpec  61017-10  HCV NS3/4A protease genotype 1b, recombinant  10 µg              
AnaSpec  72168-10  HCV NS3/4A protease genotype 3a, recombinant  10 µg             
AnaSpec  61017-5  HCV NS3/4A protease genotype 1b, recombinant  5 µg            
AnaSpec  72166-10  HCV NS3/4A protease genotype 1a, recombinant  10 µg              
AnaSpec  72169-10  HCV NS3/4A protease genotype 4d, recombinant  10 µg              
AnaSpec  72167-10  HCV NS3/4A protease genotype 2a, recombinant  10 µg              
AnaSpec  72167-5  HCV NS3/4A protease genotype 2a, recombinant  5 µg