Basement Membrane Matrix, Growth Factor Reduced (GFR), 10 ml *LDEV-Free
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產(chǎn)品英文名稱:Basement Membrane Matrix, Growth Factor Reduced (GFR), 10 ml *LDEV-Free
產(chǎn)品中文名稱:基底膜基質(zhì)膠,生長(zhǎng)因子減少,5ml*不含LDEV
BD Matrigel生長(zhǎng)因子減少(GFR)基底膜基質(zhì)膠適用于更高的所需的化學(xué)成分確定得病基底膜的制備??梢杂迷跇?biāo)準(zhǔn)的腎小球?yàn)V過率,高濃度(HC),和不含酚紅的格式中。
Basement Membrane Matrix, Growth Factor Reduced (GFR), 10 ml *LDEV-Free 產(chǎn)品介紹
BD Matrigel™ Matrix is a solubilized basement membrane preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in ECM proteins. Its major component is laminin, followed by collagen IV, heparan sulfate proteoglycan, and entactin. BD Matrigel Matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelial and other cell types.BD Matrigel Matrix is highly useful in various studies including 3D cell culture, cell invasion and migration assays,drug metabolism/toxicology, in vitro and in vivo angiogenesis assays. This report describes the use of BD Matrigel Matrix for in vivo applications such as angiogenesis and human tumor cell implantation in mice.
基底膜基質(zhì)膠,生長(zhǎng)因子減少,5ml*不含LDEV 的優(yōu)點(diǎn)
• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations are determined by Lowry method
• Endotoxin units are measured by Limulus Amoebocyte Lysate assay
• BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
Basement Membrane Matrix, Growth Factor Reduced (GFR), 10 ml *LDEV-Free 產(chǎn)品應(yīng)用
• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Basement Membrane Matrix, Growth Factor Reduced (GFR), 10 ml *LDEV-Free 保存方法
Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.
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