SeaKem? Gold Agarose
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產(chǎn)品英文名稱:SeaKem? Gold Agarose
產(chǎn)品中文名稱:SeaKem? Gold瓊脂糖粉
SeaKem? Gold Agarose瓊脂糖 產(chǎn)品介紹:
SeaKem? Gold Agarose是一種高凝膠強(qiáng)度,低EEO(電滲),標(biāo)準(zhǔn)凝膠溫度的瓊脂糖。SeaKem? Gold Agarose屬于遺傳技術(shù)級(jí)別,最適于在脈沖場(chǎng)凝膠電泳實(shí)驗(yàn)(PFGE)中快速分離巨大堿基對(duì)DNA。低電滲性能使得其在凝膠電泳實(shí)驗(yàn)中DNA電泳遷移率明顯大于使用傳統(tǒng)瓊脂糖,調(diào)節(jié)緩沖液和瓊脂糖濃度可以使跑膠時(shí)間減少一半(具體方法參照說明書)。高凝膠強(qiáng)度使其在低濃度時(shí)很容易操作,能夠使用傳統(tǒng)電泳分離大DNA片段,減少分離DNA片段時(shí)間(PFGE)。
SeaKem? Gold Agarose 產(chǎn)品參數(shù):
? Gelling temperature (1.5%) ――36°C ±1.5°C
? Melting temperature (1.5%)―― >90°C
? Gel strength (1%)―― >1,800 g/cm2
? Gel strength (1.5%)―― >3,500 g/cm2
SeaKem? Gold Agarose產(chǎn)品應(yīng)用:
? Preparative DNA and RNA electrophoresis/電泳制備DNA/RNA
? Analysis of Long PCR? reactions/長(zhǎng)PCR反應(yīng)分析
? Separation and further manipulation of DNA >1,000 bp/分離1000-50000 bp DNA
? Analytical electrophoresis of DNA and RNA /DNA和RNA分析電泳
? Blotting of DNA and RNA /DNA和RNA雜交
SeaKem? Gold Agarose 英文簡(jiǎn)述:
SeaKem? Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology Grade? Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem? Gold Agarose gels is significantly greater than in conventional agarose gels. Run times for PFGE can be decreased by as much as 50% depending upon buffer and agarose concentration. Due to its high gel strength, SeaKem? Gold Agarose forms easy-to-handle gels at low concentrations (as low as 0.5%), which allow for the separation of larger DNA fragments by conventional electrophoresis as well as a decrease in the time needed to separate DNA by PFGE. For the recovery of megabase size DNA fragments following PFGE separation, we recommend SeaPlaque? GTG? Agarose and ?-Agarase.
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