MycoAlert? Mycoplasma Detection Kit產(chǎn)品介紹：
Lonza MycoAlert Mycoplasma Detection Kit支原體檢測(cè)試劑盒是專(zhuān)門(mén)檢測(cè)細(xì)胞培養(yǎng)過(guò)程中是否被支原體污染的經(jīng)典試劑盒，它包含檢測(cè)所用到的各種試劑和產(chǎn)品操作手冊(cè)。MycoAlert?支原體檢測(cè)試劑盒檢測(cè)原理主要是：細(xì)胞培養(yǎng)中存活的支原體被溶解，然后釋放出來(lái)的酶與支原體檢測(cè)試劑盒中提供的底物發(fā)生催化反應(yīng)，將ADP轉(zhuǎn)化成ATP。通過(guò)測(cè)量樣品中添加底物前后ATP的含量，可以得到一個(gè)比率，該比率指示支原體是否存在。如果這些酶不存在，第二次讀數(shù)相對(duì)于第一次讀數(shù)就沒(méi)有增加；如果酶與底物進(jìn)行特異性反應(yīng)，就會(huì)導(dǎo)致ATP含量上升。最后通過(guò)分光光度計(jì)讀數(shù)，由于發(fā)出光的強(qiáng)度與ATP的濃度成線性關(guān)系，因此可檢測(cè)支原體的污染問(wèn)題。同時(shí)，該支原體檢測(cè)試劑盒具有操作簡(jiǎn)單、快速高效、結(jié)果穩(wěn)定可靠、靈敏度極高等優(yōu)點(diǎn)，讓您在支原體檢測(cè)時(shí)輕松容易，并確保您的培養(yǎng)潔凈無(wú)污染。

MycoAlert? Mycoplasma Detection Kit產(chǎn)品特性：
? 快速檢測(cè)：20分鐘內(nèi)即可診斷出你的細(xì)胞培養(yǎng)是否健康無(wú)污染，而其它方法一般要幾個(gè)小時(shí)到一周的時(shí)間
? 應(yīng)用方便：只需把你的細(xì)胞培養(yǎng)物與兩種試劑混合配成100μl，在分光光度計(jì)上讀2次數(shù)
? 通用性強(qiáng)：可以檢測(cè)所有支原體在細(xì)胞培養(yǎng)過(guò)程中的污染，如支原體屬、無(wú)膽甾原體屬、蟲(chóng)原體屬、脲原體屬、螺原體屬等
? 檢測(cè)靈敏：可檢測(cè)<50 cfu/ml的支原體，避免出現(xiàn)像PCR和熒光染色方法出現(xiàn)的陰性結(jié)果
? 成分完備：該支原體檢測(cè)試劑盒包含實(shí)驗(yàn)所需的所有試劑，而不像商業(yè)PCR檢測(cè)方法中需要你提供PCR酶、核酸、限制性?xún)?nèi)切酶和瓊脂糖電泳所需的其它試劑

MycoAlert? Mycoplasma Detection Kit英文簡(jiǎn)述：
The MycoAlert? Mycoplasma Detection Kit is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlert? Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert? Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert? Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

Convenient & fast
? Simply add two reagents to the culture supernatant
? Perform two luminescence readings
? Easy interpretation of results
? Results in < 20 min

Universal
? Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
? Negligible interference with media components