Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics
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Cellartis DEF-CS?500 iPS無外源3D懸浮培養(yǎng)基
傳統(tǒng)的2D培養(yǎng)系統(tǒng)無法支持放大培養(yǎng)(Scale-up)所需細(xì)胞數(shù)量級別(> 109個細(xì)胞/處理)以維持細(xì)胞療法的增長需求。?3D懸浮培養(yǎng)是大規(guī)模生產(chǎn)多能細(xì)胞的可行經(jīng)濟(jì)有效的解決方案。盡管干細(xì)胞傳統(tǒng)上難以適應(yīng)懸浮培養(yǎng),但CellArtis研究人員已經(jīng)開發(fā)出一種培養(yǎng)體系,可以在成分確定、無飼養(yǎng)層和無異源物條件下實(shí)現(xiàn)穩(wěn)定高效的生長并維持多能性。Cellartis DEF-CS?500 iPS無外源3D懸浮培養(yǎng)基與生物反應(yīng)器系統(tǒng)配套,為創(chuàng)建大量高質(zhì)量,未分化的起始材料提供了理想的方法。該培養(yǎng)基的配方基于2D培養(yǎng)基Cellartis DEF-CS 500 Xeno-Free Culture Medium,2D培養(yǎng)的細(xì)胞可以輕松過渡到3D以實(shí)現(xiàn)可靠的放大。
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英文名稱:Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics
中文名稱:Cellartis DEF-CS?500無外源3D懸浮iPS培養(yǎng)基,不含抗生素
用途:iPS?細(xì)胞3D懸浮細(xì)胞治療所需10億級
保存:基礎(chǔ)培養(yǎng)基2-8°C,添加劑-20°C
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組份
基礎(chǔ)培養(yǎng)基+三種添加劑
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)
500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No Y30046; not sold?separately)
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)
o 500 μl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)
o 200 μl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)
o 400 μl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)
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自行購買試劑
緩沖液和細(xì)胞分離試劑
PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS?–/–)
Accumax (Merck Millipore, Cat. No. SCR006)
可選抗生素和消化試劑
Gentamicin (Thermo Fisher, Cat. No. 15750)
Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)
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細(xì)胞培養(yǎng)參數(shù)
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細(xì)胞接種量 |
錐形培養(yǎng)瓶大小 |
培養(yǎng)體積 |
1–4 x 106 |
25 ml |
?5–15 ml |
3–8 x 106 |
100 ml |
16–30 ml |
7–20 x 106 |
250 ml |
35–65 ml |
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Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium
Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.
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