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產(chǎn)品編號：HY-001463

BioGems鼠β-NGF預(yù)包被ELISA試劑盒，Murine β-NGF Pre-Coated ELISA Kit

Murine β-NGF Pre-Coated ELISA Kit

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  • 生產(chǎn)廠家： BioGems
  • 訂購貨號：
  • 規(guī)格型號： 1 Kit
  • 產(chǎn)品編號： HY-001463
  • 產(chǎn)品種類： 細(xì)胞活力檢測

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產(chǎn)品描述 說明書【下載】 用戶評論【0】

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.

The BioGems Murine β-NGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure β-NGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for β-NGF. It contains NS0-expressed recombinant β-NGF. The detection antibody is a biotinylated goat polyclonal antibody specific for β-NGF. The kit is analytically validated with ready to use reagents.

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β-NGF是與BDNF，NT-3和NT-4蛋白結(jié)構(gòu)相關(guān)的神經(jīng)營養(yǎng)因子。這些蛋白屬于可能與穩(wěn)定二聚體結(jié)構(gòu)相關(guān)的半胱氨酸結(jié)家族生長因子。 β-NGF是通過其受體β-NGFR傳導(dǎo)信號的強(qiáng)效神經(jīng)營養(yǎng)因子，并且在感覺和交感神經(jīng)系統(tǒng)的發(fā)育和保存中起關(guān)鍵作用。 β-NGF還充當(dāng)B淋巴細(xì)胞的生長和分化因子，并增強(qiáng)B細(xì)胞存活。

BioGems鼠β-NGF預(yù)包被ELISA（酶聯(lián)免疫吸附試驗(yàn)）試劑盒采用固相免疫測定試劑盒，專門設(shè)計(jì)用于檢測β-NGF，其中96孔微孔板預(yù)包被β-NGF特異小鼠單克隆抗體NGF。它含有NS0表達(dá)的重組β-NGF。檢測抗體是對β-NGF特異的生物素化山羊多克隆抗體。該試劑盒經(jīng)過分析驗(yàn)證，是即用型試劑。

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Product Details

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Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<1pg/ml

Range:

31.2pg/ml-2000pg/ml

Specificity:

Natural and recombinant Murine β-NGF

Uniprot:

P01139

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 – 8 ℃.

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Components組份

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Description

Quantity

Volume

Anti-Murine β-NGF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine β-NGF Standard

2

10 ng vial

Murine β-NGF Biotinylated antibody (100x)

1

130 μL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 μL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

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Reproducibility?可重復(fù)性

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Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

136

727

1326

153

839

1645

Standard Deviation

5.3

41.44

83.54

6.58

53.7

121.73

Consistency (%CV)

3.9

5.7

6.3

4.3

6.4

7.4

窗體底端

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說明書【下載】

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