英文名稱：Recombinant Human Tumor Necrosis Factor-alpha
中文簡(jiǎn)稱：【TNF a 】重組人腫瘤壞死因子α
Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重組人腫瘤壞死因子α 細(xì)胞因子產(chǎn)品描述：
腫瘤壞死因子-α(Tumor Necrosis Factor-alpha，TNF-α)是一種主要由單核-吞噬細(xì)胞產(chǎn)生的單核因子，不僅具有選擇性地殺傷某些腫瘤細(xì)胞，而且有多種免疫調(diào)節(jié)作用。生理?xiàng)l件下具有生物學(xué)活性的人腫瘤壞死因子α（hTNF-α）是一個(gè)緊密的三聚體，其活性位點(diǎn)位于兩個(gè)相鄰亞基之間的V型結(jié)構(gòu)域，hTNF-α有兩種不同的受體，分別介導(dǎo)不同的生物學(xué)活性，在對(duì)hTNF-α結(jié)構(gòu)與功能研究的基礎(chǔ)上，人們對(duì)其N末端、C末端和p75受體結(jié)合位點(diǎn)進(jìn)行基因改造。研制了一系列衍生物，以提高h(yuǎn)TNF-α的抗腫瘤活性，降低毒副反應(yīng)。
人腫瘤壞死因子α重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有158個(gè)氨基酸，分子量為17483.77Da。該人腫瘤壞死因子α重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重組人腫瘤壞死因子α 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的人腫瘤壞死因子α(TNF a)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末中每毫克人腫瘤壞死因子是從20mM PBS（pH 7.2）,10mM NaCl溶液中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到不低于100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人腫瘤壞死因子α(TNF a )在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重組人腫瘤壞死因子α 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Introduction
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Description
Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5kDa.
The TNF-alpha is purified by standard chromatographic techniques.
Source （來(lái)源）
Escherichia Coli.
Physical Appearance （外觀）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl.
Solubility （溶解度）
It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Biological Activity （生物活性）
The Specific Activity is >5.0×107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
