英文名稱：Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor
中文簡(jiǎn)稱：【mGM CSF 】重組小鼠粒-巨噬細(xì)胞集落刺激因子

Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor 【mGM CSF 】重組小鼠粒-巨噬細(xì)胞集落刺激因子 描述：

粒-巨噬細(xì)胞集落刺激因子（granulocyte-macrophage colony-stimulating factor,GM-CSF）為造血生長(zhǎng)因子家族成員，通過細(xì)胞膜表面特異性受體（GM-CSF receptor,GMR）介導(dǎo)維持細(xì)胞存活，刺激細(xì)胞增殖與分化,，調(diào)節(jié)成熟細(xì)胞功能。人和小鼠GM-CSF分別由144和141氨基酸殘基組成，均包含17氨基酸的先導(dǎo)序列。成熟的人和小鼠GM-CSF分子分別由127和124個(gè)氨基酸殘基組成，在氨基酸水平上有54%同源性，但生物學(xué)作用具有種屬特異性。
小鼠粒-巨噬細(xì)胞集落刺激因子重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有125個(gè)氨基酸，分子量為14285.35Da。該小鼠粒-巨噬細(xì)胞集落刺激因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---大腸桿菌中重組表達(dá)

Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor 【mGM CSF 】重組小鼠粒-巨噬細(xì)胞集落刺激因子 使用須知：

溶解：提供的小鼠粒-巨噬細(xì)胞集落刺激因子（mGM CSF）產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含小鼠粒-巨噬細(xì)胞集落刺激因子（mGM CSF）的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加無菌的20mM AcOH (乙酸)以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：小鼠粒-巨噬細(xì)胞集落刺激因子 (mGM CSF )凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。

Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor 【mGM CSF 】重組小鼠粒-巨噬細(xì)胞集落刺激因子 英文描述：

Synonyms 

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.

Introduction 

GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Description 

Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.
GM-CSF Mouse is purified by proprietary chromatographic techniques.

Source 

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

GM-CSF Mouse was lyophilized with no additives.

Solubility  （溶解）

It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability   （穩(wěn)定性）

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity   （純度）

Greater than 98.0% as determined by

(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence   （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

Biological Activity  （生物活性）

The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.

Protein content  （蛋白質(zhì)含量）

GM-CSF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.