英文名稱：Recombinant Human Fibroblast Growth Factor-basic
中文簡稱：【FGF 2】重組人堿性成纖維細胞生長因子

Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重組人堿性成纖維細胞生長因子 產(chǎn)品描述：

堿性成纖維細胞生長因子（basic fibroblast growth factor，bFGF）是成纖維細胞生長因子蛋白（fibroblast growth factors，F(xiàn)GFs）家族中的一員。目前已發(fā)現(xiàn)至少22種FGF，其中N3F-1（aFGF）、FGF-2（bFGF）及FGF-7的研究較為深入。bFGF是哺乳動物和人體中一種非常微量的活性物質(zhì)，具有廣泛的生理功能和重要的臨床應用價值。bFGF不僅能刺激新生血管形成，參與創(chuàng)傷愈合和組織再生，促進胚胎組織發(fā)育和分化等，此外還與腫瘤的發(fā)生發(fā)展密切相關。近年來，重組bFGF已被應用于臨床治療創(chuàng)傷、潰瘍及神經(jīng)系統(tǒng)等疾病。
人堿性成纖維細胞生長因子重組蛋白是在大腸桿菌中重組表達的，是一條單鏈、非糖基化的多肽鏈，包含有155個氨基酸，分子量為17353Da。該人堿性成纖維細胞生長因子重組蛋白最終由標準的色譜技術純化出。
來源宿主---大腸桿菌中重組表達。

Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重組人堿性成纖維細胞生長因子 產(chǎn)品實驗須知：

溶解：提供的人堿性成纖維細胞生長因子(FGF 2)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人堿性成纖維細胞生長因子(FGF 2)的PBS（pH 7.4）溶液中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存：人堿性成纖維細胞生長因子(FGF 2)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復凍融。

Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重組人堿性成纖維細胞生長因子 產(chǎn)品英文描述：

Synonyms 

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Introduction 

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description 

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton.
The FGF-b is purified by proprietary chromatographic techniques.

Source  (來源)

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4.

Solubility 

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability  （穩(wěn)定性)

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity  （純度）

Greater than 96.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence  （氨基酸序列）

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.

Biological Activity  (生物活性）

The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg.

Protein content  （蛋白質(zhì)含量）

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.