英文名稱：Recombinant Human Brain-Derived Neurotrophic Factor
中文簡寫：【BDNF】重組人腦源性神經(jīng)營養(yǎng)因子

Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重組人腦源性神經(jīng)營養(yǎng)因子 細(xì)胞因子描述：

腦源性神經(jīng)營養(yǎng)因子（BDNF）最初從豬腦純化小分子堿性蛋白質(zhì)，是神經(jīng)營養(yǎng)因子家族一個成員，為一種小堿性蛋白質(zhì)。與神經(jīng)生長因子不同，腦源性神經(jīng)營養(yǎng)因子主要在中樞神經(jīng)系統(tǒng)，維持神經(jīng)脊和外胚層基板感覺神經(jīng)元，這些神經(jīng)元對神經(jīng)生長因子無反應(yīng)。腦源性神經(jīng)營養(yǎng)因子在神經(jīng)元存活、分化及突觸可塑性調(diào)節(jié)中起重要作用，參與了大腦學(xué)習(xí)記憶過程。BDNF生物學(xué)作用主要通過其受體TrkB介導(dǎo)。
人腦源性神經(jīng)營養(yǎng)因子重組蛋白是在大腸桿菌中重組表達(dá)的，是由兩條非糖基化多肽鏈組成的同源二聚體，包含有2 x 119個氨基酸，總分子量為26，984Da。該人腦源性神經(jīng)營養(yǎng)因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主-大腸桿菌中重組表達(dá)

Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重組人腦源性神經(jīng)營養(yǎng)因子 細(xì)胞因子實驗須知：

溶解：提供的人腦源性神經(jīng)營養(yǎng)因子(BDNF)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含人腦源性神經(jīng)營養(yǎng)因子(BDNF)的20mM 檸檬酸鈉緩沖液（pH 5）中經(jīng)透析并凍干產(chǎn)生的。我們建議在無菌的18MΩ-cm水中溶解，使其濃度不低于100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。

保存：人腦源性神經(jīng)營養(yǎng)因子(BDNF)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重組人腦源性神經(jīng)營養(yǎng)因子 細(xì)胞因子英文描述：

Synonyms 

Brain-Derived Neurotrophic Factor, BDNF, MGC34632.

Introduction 

BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Description 

Brain-Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton.
BDNF Human Recombinant is purified by proprietary chromatographic techniques.

Source 

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized WITHOUT ANY ADDITIVES.

Solubility 

It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability 

Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity 

BDNF is greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence 

The sequence of the first five N-terminal amino acids of BDNF was determined and found to be Met-His-Ser-Asp-Pro.

Biological Activity 

The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg.

Protein content 

BDNF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.