StemSep? Mouse Dendritic Cell Enrichment Kit
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產品英文名稱:Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit
產品中文名稱:Stemcell 13057 StemSep™ 小鼠樹突細胞富集試劑盒
Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit 產品簡介
Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit旨在通過陰性選擇從小鼠脾細胞或其他組織單細胞懸液中分離樹突細胞。不需要的細胞被把非樹突細胞(CD4, CD8, CD11b (Mac-1), Ly-6G/C (Gr-1), TER119),TER119)和通過生物素和葡聚糖涂層磁性顆粒識別的四聚體抗體復合物對準的生物素抗體瞄準去除。之后標記的細胞和StemSep™柱和磁鐵分離。
Stemcell 13057 StemSep™ 小鼠樹突細胞富集試劑盒產品組分
Stemcell 13057 StemSep™ 小鼠樹突細胞富集試劑盒有四個部分
• StemSep™ Mouse Dendritic Cell Enrichment Cocktail, 700 μL (5 x 700 μL)
• Anti-Biotin TAC, 2.0 mL (5 x 2.0 mL)
• StemSep™ Magnetic Colloid, 1.5 mL (5 x 1.5 mL)
• Rat Serum, 2.0 mL (5 x 2.0 mL)
Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit 推薦使用
Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit is designed for the i isolation of mouse dendritic cells from single cell suspensions of splenocytes by negative selection
Stemcell 13057 StemSep™ 小鼠樹突細胞富集試劑盒所需設備
• StemSep™ Magnet (Catalog #11030, 11050, 11060, or 11070) or a magnet with the strength of at least 0.5 Tesla
• StemSep™ Negative Selection Columns
Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit 保存方法
StemSep® Mouse DendriticCell Enrichment Cocktail and Anti-BiotinTAC
Stable at 2 - 8°C for 1 year and 2 years, respectively. Do not freeze. These products have been sterility tested.
MagneticColloid
This product is shipped at room temperature. Once opened, stable at 2 - 8°C for 6 weeks. Stable at -20°C for 1 year. Repeated freezing and thawing is possible but not recommended. Vortex before re-freezing. This product has been sterility tested.
Rat Serum
Stable at least 2 years when stored at -20°C. Stable for 2 months when stored at 2 - 8°C. This product has been sterility tested.
See Material Safety Data Sheet for more information.
Stemcell 13057 StemSep™ Mouse Dendritic Cell Enrichment Kit 實驗資料
Typical Stemsep® Mouse Dendritic Cell Enrichment Profile
Dendritic cells are defined here as CD11c+ I-A+ F4/80low. The dendritic cell content of the enriched fraction typically ranges from 30 - 45%.
Stemcell 13057 StemSep™ 小鼠樹突細胞富集試劑盒相關產品訂購信息
Stemcell 09600 StemSpan™ SFEM Serum-Free Medium for Expansion of Hematopoietic Cells
Stemcell 07915 Spleen Dissociation Medium Medium for Dissociation of Mouse Spleen
Stemcell 11000 4 Channel Peristaltic Pump StemSep™ Equipment
Stemcell 11030 StemSep™ Red Magnet with Stand Immunomagnetic Column-Based Magnet
Stemcell 11050 StemSep™ Green Magnet with Stand Immunomagnetic Column-Based Magnet
Stemcell 11060 StemSep™ Blue Magnet with Stand Immunomagnetic Column-Based Magnet
Stemcell 11070 StemSep™ Black Magnet with Stand Immunomagnetic Column-Based Magnet
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
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